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KMID : 0624620080410040328
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BMB Reports
2008 Volume.41 No. 4 p.328 ~ p.333
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Suppressive effects of Lithospermum erythrorhizon extracts on lipopolysaccharide-induced activation of AP-1 and NF-κB via mitogen-activated protein kinase pathways in mouse macrophage cells
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Han Kyu-Yeon
Kwon Taek-Hwan
Lee Tae-Hoon
Lee Sung-Joon
Kim Sung-Hoon
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Abstract
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A variety of anti-inflammatory agents have been shown to exert chemopreventive activity via targeting of transcription factors such as NF-κB and AP-1. Lithospermum erythrorhizon (LE) has long been used in traditional oriental medicine. In this study, we demonstrated the inhibitory effects of LE extracts on lipopolysaccharide (LPS)-stimulated production of inflammatory cytokines. As an underlying mechanism of inhibition, LE extracts reduced LPS-induced transactivation of AP-1 as well as NF-κB in mouse macrophage cells. Electrophoretic mobility shift assays indicated that LE extracts inhibited the DNA binding activities of AP-1 and NF-κB. In addition, phosphorylation of IκB-α protein was suppressed by LE extracts. Moreover, LE extracts inhibited c-Jun N-terminal kinase and extracellular signal-regulated signaling pathways. Our results suggest that the anti-inflammatory activity of LE extracts may be mediated by the inhibition of signal transduction pathways that normally lead to the activation of AP-1and NF-κB. These inhibitory effects may be useful for chemoprevention of cancer or other chronic inflammatory diseases. [BMB reports 2008; 41(4): 328-333]
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KEYWORD
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AP-1, Chemoprevention, Cytokines, Inflammation, Lithospermum erythrorhizon, NF-κB
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